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Evaluación de los cambios conductuales en roedores en respuesta a fármacos o cambios en el consumo de dietas. - Vinculación IPICYT

Evaluación de los cambios conductuales en roedores en respuesta a fármacos o cambios en el consumo de dietas.

Evaluación de los cambios conductuales en roedores en respuesta a fármacos o cambios en el consumo de dietas.
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Descripción

Evaluación de los cambios conductuales en roedores en respuesta a fármacos o cambios en el consumo de dietas.

Equipos utilizados en el Laboratorio de Fisiología de Ciclos Circadianos | Neurobiología y Neuropatología Molecular de Microcircuitos

1. Equipo para pruebas conductuales en roedores (puzzle box, objeto novedoso, campo abierto, motivación, etc.).
2. Equipo de biología molecular como PCR, western blot.
3. Histología, tinción de Nissl, inmunohistoquímica e inmunofluorescencia.
4. Cirugía estereotáxicaRegistro de actividad neuronal y astrocitos por miniscopes
5. Patch-clamp
6. Cultivo celular

En el laboratorio usamos una combinación de pruebas conductuales, evaluaciones histológicas, moleculares y electrofisiológicas para determinar los cambios en la relación funcional entre neuronas y astrocitos durante la inhibición de los receptores NMDA con ketamina, el consumo de una dieta alta en grasas y en la enfermedad de Huntington.

Determinar el impacto de estos modelos sobre la función entre neuronas y astrocitos, nos permitirá identificar blancos terapéuticos.

 

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